| Title | The apoptotic and anti-proliferative effects of the crude extract of Ruscus aculeatus in HTLV-1 positive and negative leukemic cells / by Maya Hussein Yassine | Thesis |
| Name(s) | Yassine, Maya Hussein (Main Author)
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| Publication | 2009
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| Link(s) | Click for full-text
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| Physical Details | x, 42 leaves; col. ill.; 30 cm.
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| Subjects | Adult T-cell leukemia
Medicinal plants--Lebanon
HTLV-I (Virus)
Cancer cells
Apoptosis
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| Classmarks | T:005290
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| Notes | Dissertation: Thesis (M.S.)--American University of Beirut, Dept. of Biology, 2009.
Dissertation: Advisor : Dr. Elias Baydoun, Professor, Department of Biology
Member of Committee : Dr. Hala Gali Muhtasib, Professor, Department of Biology
Member of Committee : Dr. Nadine Darwiche, Professor, Department of Biology.
Bib. & Index: Bibliography : leaves 39-42.
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| Abstract | Adult T- cell leukemia (ATL) is a disease resistant to chemotherapy and has a very poor prognosis where there remains no effective cure for it. It is caused by Human T-cell Lymphotropic Virus type 1 (HTLV-1).
The aim of this study is to determine the effect of the ethanol crude extract of an indigenous Lebanese plant, Ruscus aculeatus on the proliferation and apoptotic response of leukemic cells that were virus infected and also leukemic cells that were not virus infected. The Ruscus aculeatus crude extract decreased the proliferation of both types of leukemic cells in a dose- and time-dependent manner. The decrease in proliferation was detected by measuring the ability of mitochondria of metabolically active cells to convert tetrazolium salt into a blue formazan product. The Ruscus aculeatus crude extract also induced a G0/G1 cell cycle arrest and increased the level of apoptosis in the leukemic cells as confirmed by flow cytometry.
Moreover, the effect of Ruscus aculeatus crude extract on leukemic cells that were HTLV-1 positive and negative was investigated to detect the expression of several pro-apoptotic and anti-apoptotic proteins. The crude extract decreased the level of the anti-apoptotic protein Bcl-2 and increased the level of the pro-apoptotic protein p53 while the expression of the pro-apoptotic protein Bax, was not modulated. However, a significant increase in the Bax Bcl-2 ratio indicated an induction of apoptosis in those malignant cells.
This study shows that Ruscus aculeatus crude extract is favoring an anti-apoptotic cell death mechanism with the involvement of p53 protein on malignant T-cells infected with the HTLV-1 virus and malignant T-cells with no HTLV-1. The active ingredients of Ruscus aculeatus crude extract should be isolated as they may possess potential anticancer properties against ATL.
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Category | | | Jafet Archives and Special Collections | Thesis | T:5290:c.1 | ASC-Binding | Building Use |
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