| Title | Antihyperglycemic effect of Musa sapientum (banana) pseudo-stems in streptozotocin-induced diabetic rats / by Hwaida Mohammad Jaber | Thesis |
| Name(s) | Jaber, Hwaida Mohammad (Main Author)
American University of Beirut. Faculty of Arts and Sciences. Department of Biology (Related name)
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| Publication | 2009
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| Link(s) | Click for full-text
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| Physical Details | xii, 58 leaves; ill.; 30 cm.
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| Subjects | Bananas
Diabetes--Treatment
Streptozotocin
Rats--Diseases
Sodium/potassium ATPase
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| Classmarks | T:005289
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| Notes | Dissertation: Thesis (M.S.)--American University of Beirut, Dept. of Biology, 2009.
Dissertation: Advisor : Dr. Sawsan Kuraydiyyah, Professor, Biology
Member of Committee : Dr. Khuzama Knio, Professor, Biology
Member of Committee : Dr. Elias Baydoun, Professor, Biology.
Bib. & Index: Bibliography : leaves 48-58.
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| Abstract | Water extract of banana, Musa sapientum, pseudo-stems has been used as a traditional treatment for type II diabetes mellitus in Lebanon. This work aimed at verifying the claimed effect and elucidating its possible mode of action. The extract, administered in replacement of drinking water to streptozotocin (STZ)-induced diabetic rats showed a significant antihyperglycemic effect. The mechanism of action of the extract was studied by investigating its involvement in intestinal glucose absorption and its effect on the Na+/K+ ATPase and glucose transporters SGLT1 and GLUT2 in the rat jejunum. The effect of the extract on absorption was studied in rat jejuna using [Carbon 14] 3-O-methyl-D-glucose (3OMG) and an in situ perfusion technique. The activity of Na+/K+ ATPase in jejunal homogenates was assayed in vitro, by measuring the difference in the amount of inorganic phosphate release in presence and absence of enzyme inhibitors. The extract induced a significant reduction in glucose absorption and Na+/K+ ATPase activity. To investigate the involvement of the glucose transporters, the jejuna were perfused with Krebs improved buffer containing phloridzin and phloretin, respective inhibitors of SGLT1 and GLUT2. The protein expression of SGLT1 and GLUT2 was unchanged as determined by western blot analysis. The mechanism of action of the extract seems to be mediated by a reduction in the Na+/K+ ATPase activity and consequently the sodium gradient required for sugar transport by SGLT1. Reduced activity of SGLT1 leads to a decrease in intracellular glucose and in the number of apical GLUT2, which contributes to the observed antihyperglycemic effect.
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Category | | | Jafet Archives and Special Collections | Thesis | T:5289:c.1 | ASC-Binding | Building Use |
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