Title details Help Title Basic methods in protein purification and analysis: a laboratory manual / edited by Richard J. Simpson, Peter D. Adams, and Erica A. Golemis Books Name(s) Simpson, Richard J. (Related name) Adams, Peter D. (Peter David) (*Related name) Golemis, Erica (*Related name) Publication Cold Spring Harbor, N.Y. : Cold Spring Harbor Laboratory Press, c2009 Physical Details vii, 436 p.; ill.; 29 cm. Subjects Proteins--Purification--Laboratory manuals Proteins--Analysis--Laboratory manuals ISBN 9780879698676 (pbk.) Classmarks 572.6078 Notes Bib. & Index: Includes bibliographical references and index. Contents: Introduction to protein purification strategies -- Preparation of cellular and subcellular extracts -- One-dimensional polyacrylamide gel electrophoresis -- Preparative two-dimensional gel electrophoresis with immobilized pH gradients -- Ion-exchange chromatography -- Size-exclusion chromatography -- Reversed-phase high-performance liquid chromatography -- Affinity and immunoaffinity chromatography -- Metal chelate affinity chromatography -- Multidimensional chromatography of intact proteins -- Identification of protein-protein interactions by conventional column chromatography -- Identification of associated proteins by coimmunoprecipitation -- Chromatin immunoprecipitation of protein complexes -- Identification of protein-protein interactions with glutathione-s-transferase fusion proteins -- Direct visualization of protein interactions in living cells using bimolecular fluorescence complementation analysis -- In silico tools : analysis for creating focused interaction networks. Introduction to protein purification strategies -- Preparation of cellular and subcellular extracts -- One-dimensional polyacrylamide gel electrophoresis -- Preparative two-dimensional gel electrophoresis with immobilized PH gradients -- Ion-exchange chromatography -- Size-exclusion chromatography -- Reversed-phase high-performance liquid chromatography -- Affinity and immunoaffinity chromatography -- Metal chelate affinity chromatography -- Multidimensional chromatography of intact proteins -- Identification of protein-protein interactions by conventional column chromatography -- Identification of associated proteins by coimmunoprecipitation -- Chromatin immunoprecipitation of protein complexes -- Contents: Identification of associated proteins by coimmunoprecipitation -- Chromatin immunoprecipitation of protein complexes -- Identification of protein-protein interactions with glutathione-s-transferase fusion proteins -- Direct visualization of protein interactions in living cells using bimolecular fluorescence complementation analysis -- In silico tools to launch protein interaction analysis : creating focused networks around proteins of interest. Availability Barcode Location Shelf Shelfmark Status Category 00443490 Science and Agriculture Library S 572.6078:B311s:c.1 Available Standard Loan

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